Plasmid_Backbone

Part:BBa_K1480008:Design

Designed by: Roberto Alejandro Valderrama Alfaro   Group: iGEM14_UANL_Mty-Mexico   (2014-10-16)


pSB1C3 + MID, AF and BF TALEN (K1480001) Target sites


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2192
    Illegal XbaI site found at 2207
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2192
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal NotI site found at 8
    Illegal NotI site found at 2198
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2192
    Illegal XhoI site found at 1128
    Illegal XhoI site found at 2068
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2192
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2192
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2207
    Illegal SpeI site found at 1
    Illegal PstI site found at 15
    Illegal AgeI site found at 2993
    Illegal AgeI site found at 3105
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Modifications were inserted by PCR reactions in both strands.


Source

Derived from plasmid pSB1C3

References